Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: The study area. Core-genome MLST (cgMLST) profiles of B. melitensis isolates identified in this study are listed along with the study villages. The numbers in parentheses represent the number of isolates.

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques:

Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: Abortions and diagnosed B. melitensis in the Amibara district of Afar, Ethiopia.

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques:

Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: Characteristics of Brucella melitensis on culture media and gel images. ( A ) A four-day-old culture on Brucella -selective medium enriched with 5% horse serum. ( B ) bcsp31 PCR; L, 100 bp DNA molecular ladder; PC, positive control; 1, 2, 4, 6, 7, 8, 10, 12-15, Brucella spp.; 3, 5, 9, 11, and 16 negative samples; NC, negative control. ( C ) AMOS PCR; L, 100 bp DNA molecular ladder; PC, positive control; NC, negative control; No. 1–11, field isolates. ( D ) Bruce-ladder PCR; L, 1 kb-plus DNA molecular ladder; PC, positive control; NC, negative control; No. 1–11, field isolates.

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques: Positive Control, Negative Control

Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: Whole-genome SNP-based maximum likelihood phylogenetic tree of the Ethiopian B. melitensis . The figure includes the metadata of the study villages, host species from which the Brucella were isolated, and the diversity of the isolates using the different multi-locus sequence type genotyping methods. Abbreviations: MLST9/21, multi-locus sequence typing using 9- and 21-locus schemes; cgMLST, core-genome multi-locus sequence typing; rMLST, ribosomal multi-locus sequence typing; ST, sequence type; B. mel. 16M, B. melitensis biovar 1 strain 16M reference genome.

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques: Isolation, Sequencing

Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: Whole-genome SNP-based maximum likelihood phylogenetic tree of Ethiopian B. melitensis from a regional perspective. The clades: I, Western Mediterranean; II, Eastern Mediterranean; III, American; IV, African. In the figure, the B. melitensis 16M reference genome is included for comparison, and the B. abortus 2308 reference genome is used as an outgroup for rooting the phylogenetic tree. All Ethiopian isolates in the current study are labeled ETH2022-xx. Isolates that start with TE-24378* and TE.2223* were originally named 2017-TE-24378* and 2019.TE.2223*, respectively .

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques: Western Blot, Comparison, Labeling

Journal: Pathogens

Article Title: Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia

doi: 10.3390/pathogens13121063

Figure Lengend Snippet: Minimum spanning tree: ( A ) 778 B. melitensis isolates, including 24 isolates from this study based on 9-locus MLST profiles derived in silico from assembled genomes. ( B ) Twenty-one-locus MLST profiles of 616 global isolates, including those in the current study. In this figure, numbers in the bubbles represent the sequence types, and those on the lines represent branch lengths.

Article Snippet: Eighty-four publicly available B. melitensis paired-end Illumina raw sequence reads and their associated metadata from Southern Europe, the Middle East, the Americas, North Africa, and SSA were downloaded from the National Center for Biotechnology Information (NCBI) sequence reads archive (SRA) and European Nucleotide Archive (ENA) using their accession numbers (last accessed 15 July 2024).

Techniques: Derivative Assay, In Silico, Sequencing